BUSCO

Z MetaCentrum
Přejít na: navigace, hledání

Description

BUSCO provides quantitative measures for the assessment of genome assembly, gene set, and transcriptome completeness, based on evolutionarily-informed expectations of gene content from near-universal single-copy orthologs selected from OrthoDB v9.

Availability

Freely available to all users. Modules:

busco-3.0.2

Licence

MIT licence

Use

module add busco-3.0.2
run_BUSCO.py --help

Please read the documentation for Augustus, Blast+ and HMMER.

You may need to adjust environment variable AUGUSTUS_CONFIG_PATH to point to some writable directory with configurations. Eg.:

mkdir $SCRATCHDIR/augustus_configs
cp -r $AUGUSTUS_CONFIG_PATH/* $SCRATCHDIR/augustus_configs/
export AUGUSTUS_CONFIG_PATH=$SCRATCHDIR/augustus_configs
Datasets (actual to September 2018) are localized in the directory /software/busco/3.0.2/src/db/

Documentation

http://gitlab.com/ezlab/busco/raw/master/BUSCO_v3_userguide.pdf or in directory /software/busco/3.0.2/src.

Homepage

https://busco.ezlab.org/

Program manager

meta@cesnet.cz