ABySS

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Description

ABySS is a de novo, parallel, paired-end sequence assembler that is designed for short reads.

License

GNU GPL v3

Usage

Upcoming modulesystem change alert!

Due to large number of applications and their versions it is not practical to keep them explicitly listed at our wiki pages. Therefore an upgrade of modulefiles is underway. A feature of this upgrade will be the existence of default module for every application. This default choice does not need version number and it will load some (usually latest) version.

You can test the new version now by adding a line

source /cvmfs/software.metacentrum.cz/modulefiles/5.1.0/loadmodules

to your script before loading a module. Then, you can list all versions of abyss and load default version of abyss as

module avail abyss/ # list available modules
module load abyss   # load (default) module


If you wish to keep up to the current system, it is still possible. Simply list all modules by

module avail abyss

and choose explicit version you want to use.

Parallel use

Newer AbySS versions use a different way how to use parallelization. Do not run mpirun -np 8 abyss-pe. To run ABySS with 8 threads (for example), use abyss-pe np=8. The abyss-pe driver script will start the MPI process, like so: mpirun -np 8 ABYSS-P. The example with ABySS 2.3.5 (prepared as Conda environment) is following:

module add conda-modules-py37
conda activate abyss_v2.3.5_py3.6
abyss-pe np=8 k=71 B=3G name=test_run ...

Version 2.1.5 was compiled with openmpi-3.1.2-gcc!

Documentation

https://github.com/bcgsc/abyss

Homepage

http://www.bcgsc.ca/platform/bioinfo/software/abyss