FLASH

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Description

FLASH (Fast Length Adjustment of SHort reads) is a very fast and accurate software tool to merge paired-end reads from next-generation sequencing experiments. FLASH is designed to merge pairs of reads when the original DNA fragments are shorter than twice the length of reads. The resulting longer reads can significantly improve genome assemblies. They can also improve transcriptome assembly when FLASH is used to merge RNA-seq data.

License

GPLv3

Usage

Upcoming modulesystem change alert!

Due to large number of applications and their versions it is not practical to keep them explicitly listed at our wiki pages. Therefore an upgrade of modulefiles is underway. A feature of this upgrade will be the existence of default module for every application. This default choice does not need version number and it will load some (usually latest) version.

You can test the new version now by adding a line

source /cvmfs/software.metacentrum.cz/modulefiles/5.1.0/loadmodules

to your script before loading a module. Then, you can list all versions of flash and load default version of flash as

module avail flash/ # list available modules
module load flash   # load (default) module


If you wish to keep up to the current system, it is still possible. Simply list all modules by

module avail flash

and choose explicit version you want to use.

Documentation

Documentation is in file /software/flash-1.2.9/doc/README, quite explicit is also a command

flash --help

Homepage

http://ccb.jhu.edu/software/FLASH/